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KMID : 0903619960370040537
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Journal of the Korean Society for Horticultural Science
1996 Volume.37 No. 4 p.537 ~ p.543
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Sequence Analysis of PAL 4 (Phenylalanine Ammonia-lyase 4) Gene in Tomato
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이신우/Lee, Shin Woo
장영덕/여운수/Chang, Young Duck/Yeo, Yun Soo
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Abstract
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Recently, we have isolated a PALO gene fragment containing part of exon 2 region and determined its nucleotide sequences. When compared to the nucleotide sequences of tomato PAL 5 gene which encodes a normal polypeptide of 721 amino acids, PALO gene seems to produce a shorter polypeptide (about 470 amino acids) since 3´-terminal region is completely different from that of PAL 5 gene and several stop codons were observed. These prematured stop codons were also absorved from two different cultivars (Seokwang and Bonny Best) suggesting that the nucleotide sequences of PAL4 gene are quite conserved between cultivars. PALL and PAL3 gene also contain unusual stop codons but the reading frame after stop codon was not changed. Therefore, in tomato, several different size of polypeptides would be produced if they are normally translated. The result obtained from the nucleotide sequence analyses of the fragment containing such an unusual stop codon amplified from the genomic DNA of 4 different tomato cultivars (seakwang, bonny best, craigella and sweet100) by using polymerase chain reaction (PCR) suggested that such unusual stop codons were quite conserved among cultivars. In summary, our results suggest that many shorter forms of PAL enzyme can be produced due to unusual stop codons as well as shorter forms of gene and may play an important role for plant defense mechanism.
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KEYWORD
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